Core Module Information
Module title: Introductory & Applied Molecular Genetics (Hong Kong)

SCQF level: 09:
SCQF credit value: 20.00
ECTS credit value: 10

Module code: BMS09601
Module leader: Arthur Robinson
School School of Applied Sciences
Subject area group: Life Sciences

There are no pre-requisites for this module to be added

Description of module content:

Revision of structure, and function of DNA and RNA, and of the genetic code. Induction and genotypic classification of mutations, and their consequences (phenotypic effects). DNA repair (direct, excision, mismatch correction, recombination and "error-prone"). Genetic recombination, bacterial conjugation, and role of the F plasmid in gene transfer. Mapping of chromosomal markers by interrupted mating. Transformation. Transduction. Restriction enzymes and restriction mapping. Cloning DNA sequences using plasmid and bacteriophage vectors. Developments in vector technology. cDNA cloning. Identification of specific clones of interest. Analysis/manipulation of DNA sequences by: polymerase chain reaction (PCR), DNA sequencing, site-directed mutagenesis, and Southern blotting. Regulation of gene expression in prokaryotic and eukaryotic organisms.

Learning Outcomes for module:

LO1: Compare and contrast mechanisms of mutation, DNA repair, and DNA transfer in Escherichia coli, and derive genetic maps from gene transfer data.
LO2: Determine the genetic and molecular basis of restriction/modification systems and derive restriction maps by interpreting both theoretical and laboratory data.
LO3: Interpret the laboratory principles of gene cloning: the identification, selection and amplification of specific DNA sequences, and the generation of genomic libraries.
LO4: Evaluate the techniques of gene expression analysis and gene function.
LO5: Carry out laboratory transfers, manipulations and analyses of DNA.
LO6: Analyse and interpret subject-specific scientific papers.

Full Details of Teaching and Assessment

Indicative References and Reading List - URL:
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