Module title: Applications of Molecular Biology

SCQF level: 10:
SCQF credit value: 20.00
ECTS credit value: 10

Module code: MIC10105
Module leader: Amy Poole
School School of Applied Sciences
Subject area group: Biomedical Science
Prerequisites

BMS09103 Molecular Genetics

2018/9, Trimester 1, Face-to-Face, Edinburgh Napier University
Occurrence: 001
Primary mode of delivery: Face-to-Face
Location of delivery: SIGHTHILL
Partner: Edinburgh Napier University
Member of staff responsible for delivering module: Amy Poole
Module Organiser:


Learning, Teaching and Assessment (LTA) Approach:
Learning & teaching methods including their alignment to LOs
Lectures and tutorials cover LO’s 1-5. Lectures include ‘activity blocks’ during which students work in small groups to undertake exercises in experimental design, problem-solving or critiquing an article. Questioning and discussion are encouraged during face-to-face teaching in both lectures and tutorials. Considerable online resources (via Moodle) are used to support teaching and reinforce learning. LO 5 is covered by online learning activities, an interactive tutorial and the coursework assessment.

Embedding of employability/PDP/scholarship skills
Key employability skills embedded in this module include critical thinking, problem solving, written and oral communication skills and data analysis skills. Students will also become familiar with a number of online databases and software resources used routinely for the analysis of RNA, DNA and protein sequences.

Research/ teaching linkages
Students are directed (either through Moodle or during lectures/tutorials) to contemporary research literature. In tutorial sessions, students will be introduced to current/previous research conducted at Edinburgh Napier University (or other institutions) by the research-active teaching staff on the module. Staff will endeavour to link lecture topics taught on the module to their own research and specific research examples will illustrate key concepts.

Supporting equality and diversity
Materials are

Formative Assessment:
Tutorials will include elements of formative assessment, including exam technique, critical thinking and experimental design. Online resources on Moodle will contain interactive activities such as quizzes, which provide formative feedback to enable students to gauge their progress throughout the module and reinforce their learning.


Summative Assessment:
Summative assessments are in the form of a 2000-word bioinformatics-based report (LOs 4 and 5) and a 2 hour centrally timetabled exam (LO’s 1-4).


Student Activity (Notional Equivalent Study Hours (NESH))
Mode of activityLearning & Teaching ActivityNESH (Study Hours)
Face To Face Lecture 18
Face To Face Groupwork (Scheduled) 12
Online Guided independent study 12
Independent Learning Guided independent study 156
Face To Face Centrally Time Tabled Examination 2
Total Study Hours200
Expected Total Study Hours for Module200


Assessment
Type of Assessment Weighting % LOs covered Week due Length in Hours/Words
Centrally Time Tabled Examination 60 1,2,3,4 14/15 HOURS= 2, WORDS= 0
Report 40 4-5 8 HOURS= 0, WORDS= 2000
Component 1 subtotal: 60
Component 2 subtotal: 40
Module subtotal: 100

Description of module content:

The module content includes; comparative analysis of eukaryotic and prokaryotic genomes, including sequencing and content of the human genome in relation to DNA profiling and predictive medicine. You will study techniques involving manipulation of DNA and RNA for analysis of gene expression including; polymerase chain reaction (PCR), quantitative (q)PCR, RNA interference (RNAi), epigenetic, microarray and in situ hybridisation studies. You will also be introduced to in silico analysis and manipulation of RNA, DNA and protein sequence information (bioinformatics) and tutored in the use of online databases and tools for identification and comparison of DNA and protein sequences. You will examine the proteome and the different techniques involved in studying protein expression such as; two-dimensional gel electrophoresis (2-DE), sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Western blotting and other immunological techniques.

Learning Outcomes for module:

Upon completion of this module you will be able to
LO1: Analyse and interpret features of genome organisation (both prokaryotic and eukaryotic).

LO2: Compare and contrast the properties of different sequence elements within the genome and relate these to gene expression analysis, DNA profiling and predictive medicine.

LO3: Appraise a range of ‘omics’ technologies and their applications in the analysis of genomes.
LO4: Accurately assess data from DNA sequencing, microarray, PCR, in situ hybridisation and protein analysis studies.

LO5: Employ bioinformatics skills in the analysis of genomic and proteomic sequence data.

Indicative References and Reading List - URL:

Core - MOLECULAR BIOLOGY OF THE CELL 4TH EDITION ONLINE TEXTBOOK - HTTP://WWW.NCBI.NLM.NIH.GOV/BOOKS/NBK21054/
Core - THE NCBI HANDBOOK. BETHESDA (MD): NATIONAL CENTER FOR BIOTECHNOLOGY INFORMATION (US) - HTTP://WWW.NCBI.NLM.NIH.GOV/BOOKS/NBK21101/
Core - SELECTED SCIENTIFIC JOURNAL ARTICLES TO BE UPDATED ANNUALLY. ALL WILL BE AVAILABLE ON MOODLE.: VARIOUS
Recommended - BROWN, T. A (2010) GENE CLONING AND DNA ANALYSIS: AN INTRODUCTION: WILEY-BLACKWELL, 6th ed.
Recommended - BROWN, T. A. GENOMES 2. 2ND ED. - HTTP://WWW.NCBI.NLM.NIH.GOV/BOOKS/NBK21128/
Click here to view the LibrarySearch.