Module title: Cell Technology

SCQF level: 11:
SCQF credit value: 20.00
ECTS credit value: 10

Module code: MIC11103
Module leader: Rosa De Llanos
School School of Applied Sciences
Subject area group: Microbiology & Drug Discovery
Prerequisites

There are no pre-requisites for this module to be added

2019/0, Trimester 1, FACE-TO-FACE,
Occurrence: 001
Primary mode of delivery: FACE-TO-FACE
Location of delivery: SIGHTHILL
Partner:
Member of staff responsible for delivering module: Rosa De Llanos
Module Organiser:


Learning, Teaching and Assessment (LTA) Approach:
This module will be delivered through a combination of face-to-face lectures, tutorials and practical classes. All lectures and tutorial sessions (LO1-5) will include interactive components during which students will work in small groups to undertake exercises in hypotheses development and testing, project and experiment design, data-handling and report writing. Laboratory sessions will be used to develop practical skills including handling of cultures and equipment but also developing skills in working with precision and reproducibility (LO4).


Formative Assessment:
Students will engage in interactive components during lectures, tutorials and laboratory classes and be given instant feedback on their contribution.

Summative Assessment:
The summative assessment consists of two components/ a total of three elements.
Component 1 [2 elements] - The practical and laboratory component will include assessment of practical skills; verbally answering questions related to laboratory work [LOs 1-5, 3 x 30 minute assessments in week 4 - 9/ 30% weighting] and the maintenance of a lab book and writing of a laboratory report [LO 1-5, due in week 12, 30% weighting].
The second component [week 6] will assess data-handling and interpretation through a 2 hour class test [LO1, 2 & 5/ 40% weighting].


Student Activity (Notional Equivalent Study Hours (NESH))
Mode of activityLearning & Teaching ActivityNESH (Study Hours)
Face To Face Lecture 18
Face To Face Tutorial 12
Face To Face Practical classes and workshops 24
Independent Learning Guided independent study 146
Total Study Hours200
Expected Total Study Hours for Module200


Assessment
Type of Assessment Weighting % LOs covered Week due Length in Hours/Words
Class Test 30 1-5 9 HOURS= 1.5, WORDS= 0
Report 30 1-5 12 HOURS= 0, WORDS= 2500
Class Test 40 1,2,5 6 HOURS= 2, WORDS= 0
Component 1 subtotal: 60
Component 2 subtotal: 40
Module subtotal: 100

Description of module content:

Cell culture, preservation and manipulation - including microbial, plant, human/animal primary and immortalised cell lines and tissue maintenance; aseptic technique and media design; culture isolation and inoculum development, cell counting; manipulation of cells including non-recombinant and recombinant approaches to strain improvement for bioprocessing, and stem cell therapy.
Units of operation of a bioprocess and factors influencing optimisation - bioreactor design, inoculum development, scale-up, product separation and analysis.
Regulatory and quality framework - COSSH, GMP, GLP, biosafety and containment, product approval, validation and auditing. Responsible scientific practice.
Laboratory experiments/projects in cell culture, media design and preparation, optimisation of fermentation, product separation and analysis, experiment and project design, statistics, data-handling.

Learning Outcomes for module:

LO1: Appraise methods for cell culture, preservation and manipulation, including microbial cultures and animal/human cell lines.
LO2: Critically appraise the factors impacting on the optimisation of bioprocesses, including the cell culture, fermentation and product separation stages.
LO3: Critically reflect on the regulatory and quality assurance framework of relevance to bioprocessing.
LO4: Conduct, critically evaluate and present the results of laboratory experiments related to cell technology and fermentation.
LO5: Generate hypotheses and corresponding research plans including methodology and experiment design.

Indicative References and Reading List - URL:

Core - ARNOLD L DEMAIN; JULIAN E DAVIES; RONALD M. ATLAS (1999) MANUAL OF INDUSTRIAL MICROBIOLOGY AND BIOTECHNOLOGY: ASM PRESS, WASHINGTON, D.C., 2nd ed. - ISBN: 1555811280
Core - JOURNAL OF BIOTECHNOLOGY: ELSEVIER
Recommended - EL MANSI, EMT ET AL. (2007) FERMENTATION MICROBIOLOGY & BIOTECHNOLOGY: CRC/TAYLOR & FRANCIS, LONDON, 2nd ed.
Recommended - BIOLOGICALS: ELSEVIER
Recommended - HEALTH AND SAFETY EXECUTIVE - WWW.HSE.GOV.UK/
Recommended - APPLIED MICROBIAL BIOTECHNOLOGY: SPRINGER-VERLAG
Recommended - METABOLIC ENGINEERING: ELSEVIER
Recommended - NATURE: BIOTECHNOLOGY - ISSN: 10870156
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