In this module you will be introduced to various techniques and strategies used in handling microorganisms and mammalian cell lines that are important in cell technologies. This will include different methods and approaches for culturing, preserving, and manipulating various cell types including microbial, human/animal primary, and immortalised cell lines. Key aspects of cell handling will be explored including media design, isolation and purification, sterility, inoculum development, and cell quantification. We will delve into different approaches for manipulating cells and improving strains for bioprocessing, including genetic modification and stress fermentation, illustrated through case studies. You will develop an understanding of bioprocessing technologies, the units of operation and factors influencing optimisation, including bioreactor design, mode of operation and scale-up, as well as product separation and analysis. Both theoretical and applied aspects of fermentation growth kinetics will be explored, providing you with a comprehensive understanding. You will also explore commercial aspects including regulatory requirements and quality assurance processes involved in cell technologies, including COSSH, GMP, GLP, biosafety and containment, as well as product approval, validation, and auditing. Laboratory experiments and demonstrations will complement your theoretical learning and enhance your practical skills and confidence in the laboratory. Your practical sessions will cover various aspects of cell technology and bioprocessing, such as cell culture methods, media preparation, fermentation set-up, operation and monitoring, product separation and analysis, experimental design, and data-handling.